Under the auspices of our platform termed the McGill Applied Genomics Innovation Core (MAGIC), the McGill Genome Centre houses state-of-the-art equipment and multidisciplinary expertise to produce, manage and interpret large amounts of genomics data generated at low cost and with high quality.
Our supporting infrastructure includes latest sequencers from Illumina, MGI, Pacific Biosciences, Oxford Nanopore Technologies, and Life Technologies. All sequencers are supported by process automation. Most of the current commonly employed Next-Generation Sequencing (NGS) assay protocols have been introduced into the production pipelines including ChIPseq, RNAseq, Exome-seq, WGS, WGBS, and other epigenomic protocols. Standard operating procedures have been defined for all experimental steps and workflows are LIMS-controlled.
With complementary mandates from Genome Canada (supported through the Genome Technology Platform*) and the Canada Foundation for Innovation (supported through the Major Science Initiative program as a founding member of CGEn**), the McGill Genome Centre supports projects from a wide range of investigators from McGill, elsewhere in Québec and beyond.
Please feel free to contact us at pm.genome@mcgill.ca for any of your genomics needs.
*** Please note that we are currently open and accepting projects ***
| Application Area | Activity | Platform |
|---|---|---|
| Next generation sequencing | Whole genome | HiSeq X / NovaSeq 6000 |
| Exome capture | HiSeq 4000 / NovaSeq 6000 | |
| Targeted regional sequencing | HiSeq 4000 / NovaSeq 6000 | |
| Metagenomics | iSeq / MiSeq / HiSeq 4000 / NovaSeq 6000 | |
| Small genomes | PacBio Sequel | |
| Single molecule sequencing | PacBio Sequel | |
| Functional genomics & epigenomics | miRNA-seq | HiSeq 4000 / NovaSeq 6000 |
| RNA-seq | HiSeq 4000 / NovaSeq 6000 | |
| MeDIP-seq, MethylC-seq | NovaSeq 6000 | |
| ChIP-seq, ChIPmentation | NovaSeq 6000 | |
| ATAC-seq | NovaSeq 6000 | |
| Whole genome bisulfite sequencing | HiSeq X / NovaSeq 6000 | |
| Expression arrays | Affymetrix GeneTitan | |
| High throughput genotyping | Whole genome SNP arrays | Affymetrix GeneChip, GeneTitan |
| Validation studies | Amplicon sequencing | MiSeq / Ion Torrent |
| SNP genotyping | Taqman / Fluidigm SNP Type / IDT rhAmp SNP & rhAmpSeq | |
| qPCR | Roche LightCycler 480 II | |
| Single cell applications | 10x Genomics single cell applications | HiSeq 4000 / NovaSeq 6000 |
| Smartseq2 single cell RNAseq | HiSeq 4000 / NovaSeq 6000 | |
| Advanced applications | 10x Genomics linked reads | HiSeq 4000 / NovaSeq 6000 |
| Hi-C methods for genome assembly or chromatin conformation analysis | HiSeq X / NovaSeq 6000 | |
| MGI sequencing technology | DNBSEQ-G400 (previously known as MGISEQ RS2000) | |
| Nanopore sequencing technology | Oxford Nanopore PromethION | |
| Other | Nucleic acid extraction | Manual, PerkinElmer chemagic |
* The Genome Technology Platform program from Genome Canada provide researchers across Canada and internationally with access to leading-edge technologies used in genomics, metabolomics, proteomics and other related areas of research. The platforms develop new and improved technologies to support cutting-edge genomics research that improves the lives of Canadians.
** CGEn is a federally funded national platform for genome sequencing and analysis. Established in 2014, CGEn employs over 200 staff, and is funded primarily by the Canada Foundation for Innovation (CFI) through its Major Science Initiatives Fund (MSI), leveraging investments from Genome Canada and other stakeholders. CGEn operates as an integrated national platform with nodes at the McGill Genome Centre, as well as in Toronto (The Centre for Applied Genomics at The Hospital for Sick Children), and Vancouver (Canada’s Michael Smith Genome Sciences Centre), providing genomic services, including genome sequencing and analysis, that enable research in agriculture, forestry, fishery, the environment, health sciences, and many other disciplines of interest to Canadians.