The McGill Genome Centre houses state-of-the-art equipment and multidisciplinary expertise in molecular biology, genetics, epigenomics, biostatistics, population genomics, bioinformatics and other areas that are required to design genomic studies, and to produce, manage and interpret large amounts of biological data generated at low cost and with high quality.
Our supporting infrastructure includes latest sequencers from Illumina, MGI, Pacific Biosciences, Oxford Nanopore Technologies, and Life Technologies. All sequencers are supported by process automation. Most of the current commonly employed Next-Generation Sequencing (NGS) assay protocols have been introduced into the production pipelines including ChIPseq, RNAseq, Exome-seq, WGS, WGBS, and other epigenomic protocols. Standard operating procedures have been defined for all experimental steps and workflows are LIMS-controlled.
We are mandated to provide support for projects arising from the Canada Foundation for Innovation (CFI) / Major Science Initiative (MSI) / CGEn and the Genome Canada / Genomics Technology Platform.
The McGill Genome Centre is also here to support projects from external users. Please feel free to contact us for any of your genomics needs (shown in table below).
CONTACT US TO DISCUSS YOUR PROJECTS
|Next generation sequencing||Whole genome||HiSeq X / NovaSeq 6000|
|Exome capture||NovaSeq 6000|
|Targeted regional sequencing||NovaSeq 6000|
|Metagenomics||iSeq / MiSeq / NovaSeq 6000|
|Small genomes||PacBio Sequel|
|Single molecule sequencing||PacBio Sequel|
|Functional genomics & epigenomics||miRNA-seq||NovaSeq 6000|
|MeDIP-seq, MethylC-seq||NovaSeq 6000|
|ChIP-seq, ChIPmentation||NovaSeq 6000|
|Whole genome bisulfite sequencing||HiSeq X / NovaSeq 6000|
|Expression arrays||Affymetrix GeneTitan|
|High throughput genotyping||Whole genome SNP arrays||Affymetrix GeneChip, GeneTitan|
|Validation studies||Amplicon sequencing||MiSeq / Ion Torrent|
|SNP genotyping||Taqman / Fluidigm SNP Type / IDT rhAmp SNP & rhAmpSeq|
|qPCR||Roche LightCycler 480 II|
|Single cell applications||10x Genomics single cell applications||NovaSeq 6000 / DNBSEQ-G400|
|Smartseq2 single cell RNAseq||NovaSeq 6000|
|Advanced applications||10x Genomics linked reads||NovaSeq 6000|
|Hi-C methods for genome assembly or chromatin conformation analysis||HiSeq X / NovaSeq 6000|
|MGI sequencing technology||DNBSEQ-G400 (previously known as MGISEQ RS2000)|
|Nanopore sequencing technology||Oxford Nanopore PromethION|
|Other||Nucleic acid extraction||Manual, PerkinElmer chemagic|